Damaged DNA binding protein 1 (DDB1) could prevent the replication of damaged DNA, contributing to DNA repair and the maintenance of genomic integrity. These rabbit polyclonal antibodies to DDB1 (N-REKEFNKGPWKQENVE-H) were raised against a synthetic peptide derived from human p127DDB1 coupled to keyhole limpet haemocyanin (KLH). They are useful for evidencing the in vivo association of DDB1 with ROC1, a small RING finger protein that activates an ubiquitin-conjugating enzyme, and CUL4A, a cullin-dependent ligase essential to cell cycle control, genomic stability and development. This research tool is available from Kerafast and can be found at https://www.kerafast.com/product/3349/anti-ddb1-antibody.  

AALEB is an engineered cancer cell line, which was prepared from primary human bronchial epithelial cells that were serially transformed by retorvial driven expression of SV40 early region genes, human telomerase reverse transcriptase, and selection markers.

CH27 is a carcinogen-induced B-cell lymphoma tumor that also expresses IgM/k on its surface. This cell line is available for distribution from MilliporeSigma at http://www.emdmillipore.com/US/en/product/CH27-Mouse-B-Cell-Lymphoma-Cell-Line,MM_NF-SCC115.

A fibronectin receptor expressing CHO cell selected to be essentially devoid of the alpha5 subunit and that has lost its ability to attach to fibronectin, to migrate on fibronectin, or to form a fibronectin matrix. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

This cell line was prepared from the castration-recurrent human prostate cancer CWR22 xenograft. Like the CWR22 tumor, CWR-R1 cells have a functional androgen receptor with a single mutation (AR-H874Y) that has a broadened ligand specificity and is activated at low concentrations of testosterone or dihydrotestosterone (DHT). The cell line is a co-culture of epithelial and stromal fibroblasts that grow as a monolayer in defined medium based in DMEM-H. The cells can be propagated for over 50 passages and growth is increased by low levels of DHT. This cell line is available for distribution from Kerafast at https://www.kerafast.com/product/3333/human-prostate-cancer-cell-line-cwr-r1.

Antisense oligonucleotide-mediated down-regulation of genes that cause human diseases such as cancer and viral infections has been accomplished in cellular and animal models. Unfortunately, these oligonucleotides in cell culture and in vivo frequently exhibit non-antisense effects due to sequence-dependent as well as non-specific interactions with proteins. In this cell line, antisense activity of each oligomer is directly proportional to up-regulation of the EGFP reporter. This provides a positive, quantitative readout for sequence-specific antisense effects of the oligomers in the nuclei of individual cells. The signal is positive, easily detectable in individual cells, and of high sensitivity. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the LCCC Tissue Culture Facility (TCF) to inquire about availability.

This cell line expresses the P2Y4-purinergic receptor at levels that make it easier to assay. P2Y4 is expressed at the cell surface and binds adenine and uridine nucleotides to mediate cell signaling events. The receptor is expressed in a cell line that does not express other similar receptors. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

The ΔF508 mutation is a three-base pair deletion in the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) found in one out of 30 Caucasians and is the leading cause of CF. These growth-enhanced cell lines were created by introducing Bmi-1, a protooncogene that maintains stem cells, and hTERT, the catalytic subunit of telomerase, into three non-CF and three CF human bronchial epithelial cell preparations. The cell line may be used to evaluate how the molecular mechanisms which are responsible for CF result in clinical symptoms and to test pharmacological and gene therapy agents for efficacy in treating CF lung disease. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

In the human endometrium, stromal cells mediate the proliferative response of epithelial cells to the hormones estrogen and progesterone. These cells are immortalized human endometrial stromal cells and can be used in co-culture with epithelial cells to study stromal-epithelial interactions. Study of these interactions may advance our knowledge of the mechanistic, regulatory, and cell structural basis of stromal-epithelial interactions and hormonal responses in normal, and cancerous human endometrial tissue. This cell line is available for distribution from Kerafast at https://www.kerafast.com/product/3428/.

The EA.hy926 cell line was created by fusing human umbilical vein endothelial cells with carcinomic human alveolar basal epithelial cells. The resultant immortalized cell line possesses many vascular endothelial properties such as expression of Von Willebrand factor, coagulation factor VIII (anti-hemophilic factor), tissue plasminogen activator, and thrombomodulin. EA.hy926 cells grow rapidly in culture without requirement for special growth factors and possess a unique marker chromosome that makes their identification easy. These features make these cells an ideal model system for human vascular endothelial cells. EA.hy926 cells can be used to purify endothelial-specific factors, and can be used in the identification and analysis of compounds that treat endothelial-mediated pathologies such as: high/low blood pressure, blood clotting, atherosclerosis, angiogenesis and inflammation. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.