This distance-learning seminar series was created to assist those providing direct patient care with procedures which may result in acute pain. The recorded seminars review how the current situation with drug overdosing evolved and the options currently available to minimize acute pain. Most agree opioid drugs cannot be replaced entirely by clinicians’ treating acute pain. Increasingly, longer acting local anesthetics combined with anti-inflammatory drugs are reducing the need for opioid use. The discussions represent current thinking about alternatives to opioid drugs and a perspective on guidelines on opioid drug prescribing. Current data from clinical opioid drug studies conclude the sessions. The seminar series is available for licensing for educational use. All the material needed for implementing the seminar series in a course is provided (links for streaming seminar videos and a course outline). The seminar material, which contains links for streaming the seminar videos, will be available for download following acceptance of the terms of the license agreement and payment of the license fee.   Offerings Name   A Conversation About Opioids and Post-procedure Pain Control  By UNC - 22-0121

This monoclonal antibody recognizes AMPK catalytic subunits alpha 1 and alpha 2 from diverse species spanning humans to fruit flies. Possibly a useful biomarker for studies of Type 2 diabetes and Cancer or as a diagnostic tool for evaluating AMPK activity in lysates by immunoprecipitation followed by kinase assays. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes the antibodies are available from: Kerafast (ENC009 & ENC010) BIO-RAD (MCA2672 & MCA2673) Abcam (ab80039)

This monoclonal antibody binds equally well to yeast and human Cdc42: a GTPase of the Rho family that helps regulate signaling pathways by orienting the actin and secretory pathways toward a nascent bud site. This antibody can detect Cdc42 by western blot, immunoprecipitation and ELISA, which allows the measurement of Cdc42 in a vast number of studies. Unlike other methods to detect Cdc42 that subject cells to lysis reagents or require another fluorescently tagged protein, the use of this antibody allows quantitative assessment of Cdc42. This antibody was used in a novel in vivo assay to investigate the role of vesicle trafficking in the regulation of Cdc42 polarity in the publication listed below. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

Cryptochrome 1 (Cry1) and cryptochrome 2 (Cry2) proteins are core components of the mammalian circadian rhythm and help keep the circadian clocks in cells in time and in proper rhythm with the 24-hour day, as well as the seasons. Mice mutated in both genes are arrhythmic. This CRY1 hybridoma cell line is derived from Cry1-/-Cry2-/- mice, which allows investigation into cryptochrome knockout and its effects. This cell line is available for distribution from MilliporeSigma and can be found at http://www.emdmillipore.com/US/en/product/Anti-Cryptochrome-1-Antibody-clone-D46-3361,MM_NF-MABE1152#.

A mouse monoclonal antibody that will bind with the cytoskeletal protein talin. Useful primarily for research purposes to localize and study the distribution of talin inside cells. Specificity to mammalian, avian, and amphibian species. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

Noroviruses, human pathogens associated with about 85% of viral gastroenteritis, are genetically and phenotypically distinct. Divided into two large genoclusters designated GI and GII and subdivided into 8 GI genoclusters and 17 GII genoclusters, this high degree of antigenic variation complicates diagnostic and therapeutic design. These mouse monoclonal antibodies target different GI and GII strains of human noroviruses and display broad cross-reactive reactivity patterns. The antibodies may provide superior detection capabilities of more standard subsets of monoclonal antibodies. These monoclonal antibodies are available from Main Biotechnology Services at http://mainebiotechnology.com/mab227p/ and http://mainebiotechnology.com/mab228p/.

Palladin is an actin-associated protein that plays an essential role in normal cell motility. Palladin levels in breast cancer patients are higher in malignant tissues than that in normal breast samples. In addition, highly invasive breast cancer cell lines express significantly higher levels of palladin than non-invasive cell types. A mutation in the human palladin gene has been implicated in an unusual and highly penetrant form of familial pancreatic cancer. This monoclonal antibody is available from Novus Biologicals at https://www.novusbio.com/products/palladin-antibody-1e6_nbp1-25959.

GII.2.1976 Snow Mountain virus (SMV) is the prototype strain of GII.2 noroviruses (NoVs), which cause an estimated 8% of norovirus outbreaks; however, little is known about the immunobiology of these viruses. Uniquely, mouse monoclonal antibody SMV59 reacts outside the GII.2 genocluster by Western blotting and binds to a diverse panel of NoV VLPs, representing GI and GII, strains by enzyme immunoassays. SMV59 is broadly reactive and recognizes a pan-NoV, nonblockade, linear epitope, which likely resides within the shell domain of the major capsid protein. The high degree of cross-reactivity of this antibody makes it potentially useful as a diagnostic reagent. If you are an academic institution or nonprofit organization interested in this research tool for noncommercial purposes, please contact the researcher directly to inquire about availability.

Prostate cancer growth and progression depend on androgen receptor (AR) transcriptional signaling, which is increased by MAGE-A11, an AR coregulator. MAGE-A11 is a cancer-testis antigen that resides predominantly in the nucleus at low levels in normal human reproductive tract tissues and at higher levels in castration-resistant prostate cancer. These antibodies were raised in rabbits against MAGE-A11 and appear to be high affinity as low levels of MAGE-A11 can be detected. The main uses of the MAGE-A11 antibodies are western blot analysis and immunocytochemistry. These antibodies are available from Kerafast and can be found at https://www.kerafast.com/product/3332/anti-mage-a11-antibodies.

Many regulatory proteins, such as cyclins and transcription factors, are controlled by the ubiquitin-dependent proteolytic pathway. The anaphase-promoting complex (APC) is an ubiquitin ligase that plays a crucial role in regulating the passage of cells through anaphase. ROC1 and ROC2 are homologous to APC11, a subunit of APC. These rabbit polyclonal antibodies were raised specific to ROC1 and are capable of precipitating both ROC1 and the ROC1-CUL1 complex. They are useful for evidencing the in vivo association of ROC1 with cullins and potentially for the development of immunological reagents, methods and kits for detection of human cancer, and therapeutic drugs. This antibody is available for distribution from Kerafast at https://www.kerafast.com/product/3350 and Thermo Fisher Scientific at https://www.thermofisher.com/antibody/product/34-2500.